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The impact of frequently used herbicides in Argentina, on cell lines

By
Calén N. Sansalone ,
Calén N. Sansalone

1 Instituto de Biociencias, Biotecnología y Biología Traslacional (iB3), Departamento de Fisiología, Biología Molecular y Celular (DFBMC), Facultad de Ciencias Exactas y Naturales (FCEyN), Universidad de Buenos Aires (UBA), Buenos Aires, Argentina

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Facundo Couto ,
Facundo Couto

1 Instituto de Biociencias, Biotecnología y Biología Traslacional (iB3), Departamento de Fisiología, Biología Molecular y Celular (DFBMC), Facultad de Ciencias Exactas y Naturales (FCEyN), Universidad de Buenos Aires (UBA), Buenos Aires, Argentina

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Matías Blaustein ,
Matías Blaustein

2 Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina

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Mercedes García Carrillo ,
Mercedes García Carrillo

1 Instituto de Biociencias, Biotecnología y Biología Traslacional (iB3), Departamento de Fisiología, Biología Molecular y Celular (DFBMC), Facultad de Ciencias Exactas y Naturales (FCEyN), Universidad de Buenos Aires (UBA), Buenos Aires, Argentina

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Abstract

The use of herbicides worldwide is currently the subject of debate both within the scientific community and among the general public, focusing on their potential adverse effects on
human health. However, there are few studies on the molecular and cellular changes produced by herbicides.(1-5)
In this work, it was proposed to advance in the understanding of the cell signaling pathways affected by exposure to three herbicide formulations: RoundUp (RU, a.i.: glyphosate), Gesaprim (GS, a.i.: atrazine) and Paraquat Insuagro (PI, a.i.: paraquat). In particular, it was studied the effects of exposure to these formulations on the response to misfolded proteins or UPR, and the PI3K/Akt pathway in the HeLa Kyoto cell line. Dysregulation of these pathways has been reported to be associated with various malignancies.
For all in vitro herbicide exposure assays, concentrations below the recommended application limit were chosen to evaluate the effect, on different exposure times on the regulation of both pathways.(6-14) For these purposes, western blot and fluorescence microscopy techniques were used.
Results revealed activation of all three branches of the UPR (ATF6, PERK and IRE1) in those cells exposed to PI. On the other hand, ATF6 was activated as a result of GS exposure. In the case of RU, dysregulation of the PI3K/Akt pathway was observed, without detecting changes in the UPR. In conclusion, exposure of HeLa Kyoto cells to different herbicides leads to dysregulation of both the UPR and the PI3K/Akt pathway

How to Cite

1.
Sansalone CN, Couto F, Blaustein M, García Carrillo M. The impact of frequently used herbicides in Argentina, on cell lines. SCT Proceedings in Interdisciplinary Insights and Innovations [Internet]. 2024 May 3 [cited 2024 Jun. 16];2:278. Available from: https://proceedings.saludcyt.ar/index.php/piii/article/view/278

The article is distributed under the Creative Commons Attribution 4.0 License. Unless otherwise stated, associated published material is distributed under the same licence.

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